Web3.2 Purging the Guard Column Flush approximately 10–15 ml of mobile phase through a new guard column at a flow rate of 0.2–0.3 ml/minute. With resin-based HPLC columns, it is not unusual to find yellow eluant coming off the column initially for a short period. This material is polysulfonate formed during column storage. WebIf the column was used with buffered mobile phase, it is recommended that the column be flushed with HPLC grade water followed by 100% acetonitrile. Failure to flush with water may cause the mobile phase buffers to precipitate within the sorbent bed and potentially harm the column.
How to back flush and wash an HPLC column for reuse – Tips ...
WebJun 19, 2024 · I think you have injected a non-polar molecule on your column from your sample matrix. This molecule is completely retained by the column. Try reversing the column and washing it with 10x column volumes of 100% IPA. Then wash with water and finally use the manufacturer's suggested washing procedure. Re: Cleaning of HILIC … WebDetach column, and run line directly to detector, make sure the plug is not in the detector. If it is you will need to flush detector (see manufacturer recommendations on flushing detector).... how to save 30000 in a year
LC Column Cleaning and Regeneration - prep.restek.com
WebThis usually minimizes those effects if they occur. However, a word of caution: If you are using buffer salts, make sure to flush them out with at least 50% but no higher than 90% aqueous solution before gradually increasing the organic content. (If using an Aqueous C18 phase, you may use up to 100% aqueous if needed). WebDec 20, 2024 · Remove the solvent filter and prime the system. Replace the column with a union. Reverse the flow cell inlet and outlet lines. Start the flow at a very low rate, ensuring that the back pressure remains below the maximum rating (1000 psi for UV, 100 psi for RI) for the flow cell. Flush at a low flow for at least one hour. WebWash with 2 column volumes of detergent in a basic or acidic solution, e.g. 0.1–0.5% non-ionic detergent in 0.1 M acetic acid (see Table 21 for recommended flow). Rinse with 5 column volumes 70% ethanol to remove residual detergent (see Table 21 for recommended flow). Rinse with at least 2 column volumes of distilled water (same flow as step ... northern virginia carpet cleaners